《植物生理学报》 2010, 46(5): 435-440

嫁接对薄皮甜瓜果皮和果肉中乙酸己酯合成途径的影响

齐红岩*, 李金燃, 关小川

沈阳农业大学园艺学院, 辽宁省设施园艺重点实验室, 设施园艺省部共建教育部重点实验室, 沈阳 110866

通信作者：齐红岩；E-mail: hyqiaaa@126.com；Tel: 024-88487166

摘　要：

以薄皮甜瓜‘玉美人’为接穗, 进行嫁接栽培, 结果表明: 嫁接降低了花后30 d和35 d果皮中乙酸己酯含量, 对果肉中 无显著影响。在花后30 d果皮和果肉组织中分别添加乙酸己酯合成的不同阶段前体, 进行芳香物质的测定表明, 嫁接主要 降低了甜瓜果皮中己酸向乙酸己酯的转化, 而对醛和醇向酯的转化没有影响; 果肉中3种前体向乙酸己酯的转化均未受嫁 接影响。嫁接降低了花后 35 d 甜瓜果皮中脂氧合酶(LOX)、醇脱氢酶(ADH)和醇酰基转移酶(AAT)的活性, 对两个时期果 肉中相关酶活性影响不同。

关键词：嫁接; 薄皮甜瓜; 乙酸己酯; 酶活性

收稿：2010-01-06   修定：2010-03-22

资助：国家自然科学基金(30972000)、辽宁省自然科学基金(20062112)和辽宁省教育厅创新团队项目(2007T160)。

Effects of Grafting on Hexyl Acetate Biosynthetic Pathway in Peel and Flesh Tissues of Oriental Sweet Melon

QI Hong-Yan*, LI Jin-Ran, GUAN Xiao-Chuan

Key Laboratory of Protected Horticulture, Ministry of Education, Key Laboratory of Protected Horticulture of Liaoning Province,College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China

Corresponding author: QI Hong-Yan; E-mail: hyqiaaa@126.com; Tel: 024-88487166

Abstract:

The oriental sweet melon ‘Yumeiren’ was used as scion for graft cultivation. The results showed that grafting decreased the hexyl acetate content in peel tissue, and which was not affected in flesh tissue at 30th and 35th day after anthesis. The different stages of synthetic precursors were added to peel and flesh tissues at 30th day after anthesis respectively. Determination of aromatic compounds showed that grafting mainly decreased the transformation from hexanoate into hexyl acetate in peel tissue, while the transformations from aldehyde and alcohol into esters were unaffected. The transformations from three precursors into hexyl acetate were not affected in flesh tissue. Grafting decreased lipoxygenase (LOX), alcohol dehydrogenase (ADH) and alcohol acetyltransferase (AAT) activities in peel tissues of melon since 35th day after anthesis, and the enzymes showed different activities at 30th and 35th day in flesh tissues.

Key words: grafting; oriental sweet melon; hexyl acetate; enzyme activities